Yukihiro Tsuchiya ｜教員紹介 | 薬理学研究室

Profile

Name: Yukihiro Tsuchiya
Affiliation: Pharmacology
Subjects: 　
Official title: Associate Professor
Degree: Ph.D.

Academic background

2007: Ph.D., Showa Pharmaceutical University, Machida, Japan

Career history

2002 : Research Associate, Department of Microbial Chemistry, Kobe Pharmaceutical University, Kobe, Japan

2003-2007: Teaching Assistant, Showa Pharmaceutical University, Machida, Japan

2007-2010: Post-doctoral Fellow, Department of Degenerative Neurological Diseases,

National Institute of Neuroscience, National Center for Neurology and Psychiatry, Kodaira, Japan

2010-2015: Research Associate, Department of Pharmacology, Showa Pharmaceutical University, Machida, Japan

2015-2020: Assistant Professor, Department of Pharmacology, Showa Pharmaceutical University, Machida, Japan

2020- : Associate Professor, Department of Pharmacology, Showa Pharmaceutical University, Machida, Japan

Licenses and qualifications

License for Pharmacist
Public Health Laboratory Technologist

Honors & Awards

2004 Presentation Award, Pharma-Bio Forum 2004, Division of Pharma-Biology, The Pharmaceutical Society of Japan

Academic societies

Nitric Oxide Society

The Pharmaceutical Society of Japan

The Japanese Biochemical Society

The Nitric Oxide Society of JAPAN

The Japanese Pharmacological Society

Society for Free Radical Research Japan

Research fields

Post-translational modification of proteins

Books

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日本薬理学雑誌151巻6号　土屋幸弘　Vox Novaリレーエッセイ　授業と翻訳後修飾と、時々、フルート　273　日本薬理学会　2018　0015-5691
生体の科学　増大特集　タンパク質?核酸の分子修飾　2018年10月発行 (増大号) (Vol.69 No.5)　高田剛?土屋幸弘?渡邊泰男　酸化還元状態　Sーグルタチオン化　442-443　医学書院（東京）　2018　4910055551081
JOHNS31巻8号　土屋幸弘　自律神経薬概論　1045-1048　東京医学社　2015　978-4-88563-374-4

Papers

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[Original Paper]　Discovery of a cystathionine γ-lyase (CSE) selective inhibitor targeting active-site pyridoxal 5'-phosphate (PLP) via Schiff base formation.　Echizen H., Hanaoka K., Shimamoto K., Hibi R., Toma-Fukai S., Ohno H., Sasaki E., Komatsu T., Ueno T., Tsuchiya Y., Watanabe Y., Otsuka T., Saito H., Nagatoishi S., Tsumoto K., Kojima H., Okabe T., Shimizu T., Urano Y.　Sci Rep　13　16456　2023　[Related links]
[Original Paper] 　Cystathionine γ-Lyase Self-Inactivates by Polysulfidation during Cystine Metabolism　Araki S., Takata T., Ono K., Sawa T., Kasamatsu S., Ihara H., Kumagai Y., Akaike T., Watanabe Y., Tsuchiya T.　Int. J. Mol. Sci. 　24　9982　2023　[Related links]
[Review]　Oxidative Stress Orchestrates MAPK and Nitric-Oxide Synthase Signal　Takata T., Araki S., Tsuchiya Y., Watanabe Y.　Int. J. Mol. Sci.　21　8750　2020　[Related links]
[Review](Cover image: Related links)　Coordination between Calcium/Calmodulin-Dependent Protein Kinase II and Neuronal Nitric Oxide Synthase in Neurons　Araki S., Osuka K., Takata T., Tsuchiya Y., Watanabe Y.　Int. J. Mol. Sci.　21　7997　2020　[Related links]
[Review]　Persulfide signaling in stress-initiated CaM kinase response.　Takata T., Araki S., Tsuchiya Y., Watanabe Y.　Antioxid. Redox Signal.　33　1308-1319　2020　[Related links]
[Original Paper]　The active-site cysteine residue of Ca2+/calmodulin-dependent protein kinase I is protected from irreversible modification via generation of polysulfidation.　Takata T., Tsukuda A., Tsuchiya Y., Akaike T., Watanabe Y.　Nitric Oxide　86　68–75　2019　[Related links]
[Original Paper]　Reactive sulfur species impair Ca2+/calmodulin-dependent protein kinase II via polysulfidation.　Araki S., Takata T., Tsuchiya Y., Watanabe Y.　Biochem. Biophys. Res. Commun.　508　550-555　2019　[Related links]
[Original Paper]　Redox regulation of Ca2+/calmodulin-dependent protein kinase IV via oxidation of its active-site cysteine residue.　Takata T., Kimura J., Ihara H., Hatano N., Tsuchiya Y., Watanabe Y.　Free Radic. Biol. Med.　130　99-106　2019　[Related links]
[Original Paper]　1,4-Naphthoquinone activates the HSP90/HSF1 pathway through the S-arylation of HSP90 in A431 cells: Negative regulation of the redox signal transduction pathway by persulfides/polysulfides.　 Abiko Y., Sha L., Shinkai Y., Unoki T., Luong N. C., Tsuchiya Y., Watanabe Y., Hirose R., Akaike T., Kumagai Y. 　Free Radic Biol Med. 　104　118-128. 　2017　[Related links]
[Original Paper]　Discovery and Mechanistic Characterization of Selective Inhibitors of H2S-producing Enzyme: 3-Mercaptopyruvate Sulfurtransferase (3MST) Targeting Active-site Cysteine Persulfide. 　Hanaoka K., Sasakura K., Suwanai Y., Toma-Fukai S., Shimamoto K., Takano Y., Shibuya N., Terai T., Komatsu T., Ueno T., Ogasawara Y., Tsuchiya Y., Watanabe Y., Kimura H., Wang C., Uchiyama M., Kojima H., Okabe T., Urano Y., Shimizu T., Nagano T..　Sci Rep. 　7 　40227. 　2017　[Related links]
[Original Paper]　Reactive sulfur species inactivate Ca2+/calmodulin-dependent protein kinase IV via S-polysulfidation of its active-site cysteine residue. 　Takata T., Ihara H., Hatano N., Tsuchiya Y., Akaike T., Watanabe Y. 　Biochem. J. 　474　2547-2562. 　2017　[Related links]
[Original paper]　Formation of sulfur adducts of N-acetyl-p-benzoquinoneimine, an electrophilic metabolite of acetaminophen in vivo: participation of reactive persulfides.　Abiko Y., Ishii I., Kamata S., Tsuchiya Y., Watanabe Y., Ihara H., Akaike T., Kumagai Y.　Chem. Res. Toxicol.　28　1796-1802　2015　[Related links]
[Original paper]　Reactive cysteine persulfides and S-polythiolation regulate oxidative stress and redox signaling.　Ida T., Sawa T., Ihara H., Tsuchiya Y., Watanabe Y., Kumagai Y., Suematsu M., Motohashi H., Fujii S., Matsunaga T., Yamamoto M., Ono K., Devarie-Baez N. O., Xian M., Fukuto J. M., Akaike T.　Proc. Natl. Acad. Sci. U. S. A.　111　7606-7611　2014　[Related links]
[Original paper]　TDP-43 associates with stalled ribosomes and contributes to cell survival during cellular stress.　Higashi S., Kabuta T., Nagai Y., Tsuchiya Y., Akiyama H., Wada K.　J. Neurochem.　126　288-300　2013　[Related links]
[Original paper]　Ubiquitin C-terminal hydrolase L1 (UCH-L1) acts as a novel potentiator of cyclin-dependent kinases to enhance cell proliferation independently of its hydrolase activity.　Kabuta T., Mitsui T., Takahashi M., Fujiwara Y., Kabuta C., Konya C., Tsuchiya Y., Hatanaka Y., Uchida K., Hohjoh H., Wada K.　J. Biol. Chem.　288　12615-12626　2013　[Related links]
[Original paper]　90-kDa ribosomal S6 kinase 1 is inhibited by S-glutathionylation of its active-site cysteine residue during oxidative stress.　Takata T., Tsuchiya Y., Watanabe Y.　FEBS Lett.　587　1681-1686　2013　[Related links]
[Original paper]　Nitric oxide enhances increase in cytosolic Ca2+ and promotes nicotine-triggered MAPK pathway in PC12 cells.　Kajiwara A., Tsuchiya Y., Takata T., Nyunoya M., Nozaki N., Ihara H., Watanabe Y.　Nitric Oxide　34　 3-9　2013　[Related links]
[Original paper]　Calcium/calmodulin-dependent protein kinases as potential targets of nitric oxide.　Takata T., Kimura J., Tsuchiya Y., Naito Y., Watanabe Y.　Nitric Oxide　25　145-152　2011　[Related links]
[Original paper]　4-Hydroxy-2-nonenal-modified glyceraldehyde-3-phosphate dehydrogenase is degraded by cathepsin G in rat neutrophils.　Tsuchiya Y., Okada G., Kobayashi S., Chikuma T., Hojo H.　Oxid. Med. Cell Longev.　　2011:213686　2011　[Related links]
[Original paper]　Nitric oxide promotes nicotine-triggered ERK signaling via redox reactions in PC12 cells.　Miyamoto Y., Sakai R., Maeda C., Takata T., Ihara H., Tsuchiya Y., Watanabe Y.　Nitric Oxide　25　344-349　2011　[Related links]
[Original paper]　Skeletal muscles of Uchl3 knockout mice show polyubiquitinated protein accumulation and stress responses.　Setsuie R., Suzuki M., Tsuchiya Y., Wada K.　Neurochem. Int.　56　911-918　2010　[Related links]
[Original paper]　TDP-43 physically interacts with amyotrophic lateral sclerosis-linked mutant CuZn superoxide dismutase.　Higashi S., Tsuchiya Y., Araki T., Wada K., Kabuta T.　Neurochem. Int.　57　906-913　2010　[Related links]
[Original paper]　Facile synthesis and in vitro properties of 1-alkyl- and 1-alkyl-N-propargyl-1,2,3,4-tetrahydroisoquinoline derivatives on PC12 cells.　Kitabatake M., Nagai J., Abe K., Tsuchiya Y., Ogawa K., Yokoyama T., Mohri K., Taguchi K., Horiguchi Y.　Eur. J. Med. Chem.　44　4034-4043　2009　[Related links]
[Original paper]　Familial amyotrophic lateral sclerosis-linked mutant SOD1 aberrantly interacts with tubulin.　Kabuta T., Kinugawa A., Tsuchiya Y., Kabuta C., Setsuie R., Tateno M., Araki T., Wada K.　Biochem. Biophys. Res. Commun.　387　121-126　2009　[Related links]
[Original paper]　4-Hydroxy-2-nonenal-modified glyceraldehyde-3-phosphate dehydrogenase is degraded by cathepsin G.　Tsuchiya Y., Okuno Y., Hishinuma K., Ezaki A., Okada G., Yamaguchi M., Chikuma T., Hojo H.　Free Radic. Biol. Med.　43　1604-1615　2007　[Related links]
[Original paper]　Axonal transports of tripeptidyl peptidase II in rat sciatic nerves.　Chikuma T., Shimizu M., Tsuchiya Y., Kato T., Hojo H.　Neurochem. Int.　50　236-242　2006　[Related links]
[Original paper]　Degradation of glyceraldehyde-3-phosphate dehydrogenase triggered by 4-hydroxy-2-nonenal and 4-hydroxy-2-hexenal.　Tsuchiya Y., Yamaguchi M., Chikuma T., Hojo H.　Arch. Biochem. Biophys.　438　217-222　2005　[Related links]
[Original paper]　Preparation of monoclonal antibodies reactive to a hallucinogenic drug, psilocin.　Yamaguchi M., Saito T., Horiguchi Y., Ogawa K., Tsuchiya Y., Hishinuma K., Chikuma T., Makino Y., Hojo H.　J. Health Sci.　50　600-604　2004　[Related links]
[Original paper]　Conformational change of glyceraldehyde-3-phosphate dehydrogenase induced by acetylleucine chloromethyl ketone is followed by unique enzymatic degradation.　Yamaguchi M., Tsuchiya Y., Hishinuma K., Chikuma T., Hojo H.　Biol. Pharm. Bull.　26　1648-1651　2003　[Related links]
[Original paper]　Degradation of glyceraldehyde-3-phosphate dehydrogenase induced by acetylleucine chloromethyl ketone in U937 cells.　Yamaguchi M., Tsuchiya Y., Chikuma T., Hojo H.　Biochem. Pharmacol.　63　1857-1862　2002　[Related links]

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Conference, etc.

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[Poster presentation] 　The redox response of sulfur transferase to nitrosothiols　Yukihiro Tsuchiya, Shoma Araki, Yasuo Watanabe　The 95th Annual Meeting of the Japanese Biochemical Society　Nagoya　2022/11/9?2022/11/11
[Poster presentation]　Neuronal nitric oxide synthase is regulated by supersulfides　Yukihiro Tsuchiya, Shoma Araki, Yasuo Watanabe　The 96th Annual Meeting of the Japanese Pharmacological Society　Yokohama　2022/11/30?2022/12/3
[Symposium (Invited Speaker)]　Regulation of neuronal nitric oxide synthase by supersulfides 　Yukihiro Tsuchiya, Shoma Araki, Yasuo Watanabe　Redox Week in Sendai 2022　Sendai　2022/10/28?2022/11/1
[Oral presentation]　Regulation of neuronal NO synthase uncoupling via reactive sulfur species　Yukihiro Tsuchiya, Shoma Araki, Yasuo Watanabe　The 74th Annual Scientific Meeting of the Society for Free Radical Research JAPAN, The 21st Annual Scientific Meeting of the Nitric Oxide Society of Japan　Live-Web　2021/5/19?2021/5/20
[Oral presentation]　Cystathionine γ-lyase as a redox sensor　Yukihiro Tsuchiya, Shoma Araki, Yasuo Watanabe　The 73rd Annual Scientific Meeting of the Society for Free Radical Research JAPAN, The 20th Annual Scientific Meeting of the Nitric Oxide Society of Japan　Yonago　2020/10/6?10/7
[Poster presentation]　Acetylcholine signal in PC12 cells and nobilerin as its inhibitor　Yukihiro Tsuchiya, Riko Matsushita, Tsuyoshi Takata, Hideshi Ihara, Yasuo Watanabe　The 19th Annual Scientific Meeting of the Nitric Oxide Society of Japan　Kurume　2019/6/14?2019/6/15
[Poster presentation]　Effect of nobiletin on acetylcholine signaling　Yukihiro Tsuchiya, Riko Matsushita, Tsuyoshi Takata, Hideshi Ihara, Yasuo Watanabe　The 91st Annual Meeting of the Japanese Biochemical Society　Kyoto　2018/9/24?2018/9/26
[Poster presentation]　NEURONAL NOS EXPRESSION PROMOTES ACETYLCHOLINE RECEPTOR SIGNALING ENHANCEMENT　Yukihiro Tsuchiya, Tsuyoshi Takata, Hideshi Ihara, Yasuo Watanabe　The 10th International Conference on the Biology, Chemistry and Therapeutic Applications of Nitric Oxide　Oxford　2018/9/16-2018/9-20
[Poster presentation]　Mechanism of nobiletin as an acetylcholine antagonist　Yukihiro Tsuchiya, Riko Matsushita, Tsuyoshi Takata, Hideshi Ihara, Yasuo Watanabe　The 71st Annual Scientific Meeting of the Society for Free Radical Research JAPAN, The 18th Annual Scientific Meeting of the Nitric Oxide Society of Japan　Kyoto　2018/5/17?2018/5/18
[Poster presentation]　Significance of Redox Modification by NOS in Acetylcholine Receptor Signaling Pathway　Yukihiro Tsuchiya, Ayumi Ide, Megumi Naito, Natsumi Tanzawa, Tsuyoshi Takata, Hideshi Ihara, Yasuo Watanabe　ConBio2017　Kobe　2017/12/6?2017/12/9
[Poster presentation]　Effects of NO-synthase and its Ser⁸⁴⁷ phosphorylation in nicotine -triggered cell signaling　Yukihiro Tsuchiya, Tanzawa Natsumi, Tsuyoshi Takata, Hideshi Ihara, Yasuo Watanabe　The 17th Annual Scientific Meeting of the Nitric Oxide Society of Japan　Tokushima　2017/05/19～2017/05/20
[Poster presentation]　Ca²⁺ influx enhancement via TRPC activation in NOS-expressing cells　Yukihiro Tsuchiya, Megumi Naito, Riko Matsushita, Mayumi Nyunoya, Aya Kajiwara , Tsuyoshi Takata, Hideshi Ihara, Yasuo Watanabe　The 89th Annual Meeting of the Japanese Biochemical Society　Sendai　2016/9/25～2016/9/27
[Special lecture/Symposium]　NOS発現細胞におけるニコチン作動性Ca²⁺流入メカニズム　Yukihiro Tsuchiya　The 60th Kanto Branch Meeting, The Pharmaceutical Society of Japan　Tokyo　2016/9/17
[Poster presentation]　Involvement of TRPC in nicotine-induced Ca²⁺ influx in nNOS-expressed PC12 cells　Yukihiro Tsuchiya, Tsuyoshi Takata, Hideshi Ihara, Yasuo Watanabe　The 9th International Conference on the Biology, Chemistry, and Therapeutic Application of Nitric Oxide, The 16th Annual Scientific Meeting of the Nitric Oxide Society of Japan　Sendai　2016/05/20～2016/05/22
[Poster presentation]　TRPC活性化を介するNOによる細胞内Ca²⁺流入制御　Yukihiro Tsuchiya, Megumi Naito, Riko Matsushita, Mayumi Nyunoya, Aya Kajiwara , Tsuyoshi Takata, Hideshi Ihara, Yasuo Watanabe　The 89th Annual Meeting of the Japanese Pharmacological Society　Yokohama　2016/03/09～2016/03/11
[Poster presentation]　NOによる細胞内Ca²⁺流入増強におけるTRPCチャネルの関与　Yukihiro Tsuchiya, Megumi Naito, Riko Matsushita, Mayumi Nyunoya, Aya Kajiwara , Tsuyoshi Takata, Hideshi Ihara, Yasuo Watanabe　The 15th Annual Scientific Meeting of the Nitric Oxide Society of Japan　Toyonaka　2015/06/26～2015/06/27
[Poster presentation]　ニコチンにより誘導されるCa²⁺流入におけるNOの関与　Yukihiro Tsuchiya, Mayumi Nyunoya, Aya Kajiwara , Tsuyoshi Takata, Hideshi Ihara, Yasuo Watanabe　The 88th Annual Meeting of the Japanese Pharmacological Society　Nagoya　2015/03/18～2015/03/20
[Special lecture/Symposium]　生体内活性イオウ合成系とレドックス制御　Yukihiro Tsuchiya　Symposium of Grant-in-Aid for Scientific Research (S) Comprehensive study on environmental electrophiles- mediated signal transduction pathways regulated by reactive sulfur species　Tsukuba　2014/10/27
[Oral presentation]　NOによる細胞内Ca²⁺流入調節機構の解析　Yukihiro Tsuchiya, Mayumi Nyunoya, Aya Kajiwara, Haruka Sato, Hideshi Ihara, Yasuo Watanabe　The 131th Kanto Regional Meeting, The Japanese Pharmacological Society　Yokohama　2014/10/11
[Poster presentation]　Role of nNOS expression in nicotine-induced cytosolic Ca²⁺ influx in PC12 cells.　Yukihiro Tsuchiya, Mayumi Nyunoya, Aya Kajiwara, Hideshi Ihara, Yasuo Watanabe　The 8th International Conference on the Biology, Chemistry and Therapeutic Application of Nitric Oxide　Cleveland　2014/06/16～2014/06/20
[Oral presentation]　NOによる細胞内Ca²⁺流入増強メカニズムの解明　Yukihiro Tsuchiya, Mayumi Nyunoya, Aya Kajiwara, Hideshi Ihara, Yasuo Watanabe　The 14th Annual Scientific Meeting of the Nitric Oxide Society of Japan　Saga　2014/05/16～2014/05/17
[Poster presentation]　NOによるp38 MAPKリン酸化シグナルの増強とそのカルシウムの関与　Yukihiro Tsuchiya, Aya Kajiwara, Mayumi Nyunoya, Hideshi Ihara, Yasuo Watanabe　The 13th Annual Scientific Meeting of the Nitric Oxide Society of Japan　Okinawa　2013/06/28～2013/06/29
[Special lecture/Symposium]　生体内sulfur transferaseの酵素反応メカニズムについて　Yukihiro Tsuchiya, Yasuo Watanabe　The 66th Annual Meeting of Society for Free Radical Research Japan　Nagoya　2013/06/13～2013/06/14
[Poster presentation]　CaMKIIおよびPKCによる硫化水素産生酵素CBSのリン酸化について　Yukihiro Tsuchiya, Tatsuya Kuno, Kaoru Yamada, Naoya Hatano, Norihiro Shibuya, Hideo Kimura, Yasuo Watanabe　The 85th Annual Meeting of the Japanese Biochemical Society　Fukuoka　2012/12/14～2012/12/16
[Poster presentation]　Reversible inactivation of Ca²⁺/calmodulin-dependent protein kinase IV by oxidation of the active-site cysteine residue　Yukihiro Tsuchiya, Jun Kimura, Yasuo Watanabe　The 7th International Conference on the Biology, Chemistry and Therapeutic Application of Nitric Oxide　Edinburgh　2012/07/22～2012/07/26
[Oral presentation]　レドックスによるカルモデュリンキナーゼIVの活性制御　Yukihiro Tsuchiya, Jun Kimura, Yasuo Watanabe　The 126th Kanto Regional Meeting, The Japanese Pharmacological Society　Tokyo　2012/07/14
[Poster presentation]　硫化水素産生酵素のCaMKII、PKA、PKCによるリン酸化修飾の検討　Yukihiro Tsuchiya, Naoya Hatano, Tatsuya Kuno, Kaoru Yamada, Yasuo Watanabe　The 12th Annual Scientific Meeting of the Nitric Oxide Society of Japan　Kobe　2012/06/29～2012/06/30
[Poster presentation]　カルモデュリンキナーゼIVはレドックスにより活性が制御される　Yukihiro Tsuchiya, Jun Kimura, Yasuo Watanabe　The 85th Annual Meeting of the Japanese Pharmacological Society　Kyoto　2012/03/14～2012/03/16
[Poster presentation]　マウス海馬でのニコチンによるERK活性化におけるNOの関与　Yukihiro Tsuchiya, Chiharu Maeda, Yoshiaki Miyamoto, Hideshi Ihara , Yasuo Watanabe　The 11th Annual Scientific Meeting of the Nitric Oxide Society of Japan　Machida　2011/05/13～2011/05/14

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Activities in society

2016- Board of Council Members of the Nitric Oxide Society of JAPAN

2018- Board of Council Members of the Japanese Pharmacological Society

Yukihiro TsuchiyaAssociate Professor / Ph.D.last update : 2024.07.26

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Licenses and qualifications

Honors & Awards

Academic societies

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Books

Papers

Conference, etc.

Activities in society